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What Is Transglutaminase?

How the Meat-Binding Enzyme Works and Wholesale Sourcing Guide

How transglutaminase cross-links proteins, usage rates and reaction conditions in meat, dairy and plant-based applications, regulation and labelling, wholesale buying criteria.

Reading time: 3 min Transglutaminase →
In this guide
What is transglutaminase? How does it work? Reaction conditions Applications and typical rates Regulation and labelling Technical properties and quality criteria Wholesale sourcing: price, storage and minimum order Frequently asked questions
Transglutaminase
Processing Aids Transglutaminase Product page and quote
01

What is transglutaminase?

Transglutaminase (TG) is an enzyme that forms covalent cross-links between glutamine and lysine residues in proteins. The commercial form used in the food industry is produced by fermentation of Streptomyces mobaraensis and is called microbial transglutaminase (mTG). It is popularly known as "meat glue", but its function goes beyond binding pieces: it improves texture and water holding in any protein system.

02

How does it work?

The enzyme forms an isopeptide bond between the amide group of glutamine in one protein chain and the amino group of lysine in another. This bond resists heat and enzymatic breakdown and does not reverse once formed. The proteins build a network: meat pieces can be sliced as one, sausage emulsions become firmer, yoghurt releases less whey and plant-based burgers hold together.

03

Reaction conditions

The optimum temperature is 40–55 °C and optimum pH 5–8; in industry, however, the reaction is usually run in the cold chain (0–5 °C) over 2–24 hours because microbiological safety comes first. The enzyme is inactivated above 70 °C, so no active enzyme remains in a cooked product. Even distribution is critical: the powder is added to a dry mix or as a solution prepared with cold water.

04

Applications and typical rates

Restructured meat and binding of meat pieces (0.5–1%); slice strength in sausages, cold cuts and ham (0.3–0.7%); gel strength in surimi and fish products (0.2–0.5%); texture and reduced whey separation in yoghurt and cheese (0.01–0.05%); binding in plant-based burgers and meat alternatives (0.3–1%); elasticity in noodles and pasta. Because commercial preparations differ in activity, always calculate the dose from activity (U/g).

05

Regulation and labelling

Transglutaminase is treated as a food enzyme. Under Turkish and EU regulations enzymes are generally considered processing aids and need not be declared when they have no function in the finished product; however, products that join meat pieces into a whole-cut appearance require wording such as "formed meat" in the product name so consumers are not misled. Always check target market regulations for your product category.

06

Technical properties and quality criteria

Check enzyme activity (U/g; typically 80–120), carrier composition (maltodextrin, caseinate, salt), moisture, particle size, microbiological values, heavy metals, allergen statement (milk allergen if the carrier contains caseinate) and non-GMO statement. Preparations carried on caseinate require a milk allergen declaration; ask for a caseinate-free grade for plant-based products.

07

Wholesale sourcing: price, storage and minimum order

The wholesale price of transglutaminase is set by enzyme activity, carrier type, origin (Japan and China are common) and order quantity. Because activity declines over time, cold storage (≤ 10 °C) and a recently produced lot matter. The typical minimum order is 100–500 kg. PERDIST identifies the preparation suited to your application, compares sources and provides a written quotation with documents.

08

Frequently asked questions

Food-grade microbial transglutaminase is a food enzyme assessed by the competent authorities and it is inactivated during cooking. Use must follow the permitted rates and labelling rules.
Many commercial preparations use sodium caseinate as carrier, which requires a milk allergen declaration and cannot be used in vegan products. Ask for a maltodextrin-carried grade for plant-based applications.
If the dose stays the same, binding weakens. Check the activity value of every new lot and recalculate the dose accordingly.

Need current pricing, minimum order, origin or technical documents for this ingredient? Tell us the application and monthly quantity; we reply with a written quotation within one business day.

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